PrimePCR™ SYBR® Green Assay: Dcaf11, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0055205

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Dcaf11, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Dcaf11, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0046779
Assay Design:   exonic
Chromosome Location:   14:55561265-55561408question
Amplicon Length:   114
Splice Variants Targeted:   ENSMUST00000110637 ENSMUST00000110638 ENSMUST00000062658 ENSMUST00000117236 ENSMUST00000147981 ENSMUST00000133256 ENSMUST00000121622 ENSMUST00000143431 ENSMUST00000150481 ENSMUST00000072530 ENSMUST00000128490 ENSMUST00000117701

Gene Information

Description Not Available

Gene Symbol:   Dcaf11
Gene Name:   DDB1 and CUL4 associated factor 11
Aliases:   0710008A13Rik, C76035, D14Ucla1, Dacf11, GLO14, Wdr23
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000022214
Entrez:   28199
Chromosome Mapping:   14 C3|14 24.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.110000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download