PrimePCR™ SYBR® Green Assay: Cideb, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best negative control; Same primer pair as used in probe assay qMmuCEP0054698

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0046272
Assay Design:   exonic
Chromosome Location:   14:55758257-55758403question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000001497

Gene Information

Description Not Available

Gene Symbol:   Cideb
Gene Name:   Cell death-inducing DNA fragmentation factor, alpha subunit-like effector B
Aliases:   1110030C18Rik, AI790179, CIDE-B
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000022219
Entrez:   12684
UniGene:   Mm.466766
Chromosome Mapping:   14|14 C1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.370000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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