This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCEP0054676
PrimePCR™ PreAmp for SYBR® Green Assay: Cartpt, Mouse
PrimePCR™ Template for SYBR® Green Assay: Cartpt, Mouse
This gene encodes a preproprotein that is processed into multiple biologically active small secreted peptides. Expression of this gene is regulated by cocaine and other drugs and is associated with feeding/appetite and stress response. Alternatively spliced transcript variants have been observed encoding different protein isoforms each of which are subsequently processed into CART I and CART II mature peptides. [provided by RefSeq Oct 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.