PrimePCR™ SYBR® Green Assay: Cant1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0053979

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0045553
Assay Design:   exonic
Chromosome Location:   11:118408883-118410999question
Amplicon Length:   119
Splice Variants Targeted:   ENSMUST00000017620 ENSMUST00000092378 ENSMUST00000106289 ENSMUST00000106287 ENSMUST00000106288 ENSMUST00000164927

Gene Information

The protein encoded by this gene is a calcium-dependent nucleotidase that preferentially hydrolyzes UDP GDP and IDP. The encoded protein has low activity with ADP and ATP and shows no activity with AMP and GMP. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jun 2012]

Gene Symbol:   Cant1
Gene Name:   Calcium activated nucleotidase 1
Aliases:   5830420C20Rik, Apy1h, D11Bwg0554e, Entpd8, SCAN-1, Shapy
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000025575
Entrez:   76025
UniGene:   Mm.177551
Chromosome Mapping:   11 E2|11 75.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.500000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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