PrimePCR™ SYBR® Green Assay: Cog1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0053867

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0045441
Assay Design:   exonic
Chromosome Location:   11:113659292-113660950question
Amplicon Length:   64
Splice Variants Targeted:   ENSMUST00000128119 ENSMUST00000112070 ENSMUST00000026740 ENSMUST00000011895 ENSMUST00000172269 ENSMUST00000018805 ENSMUST00000063776 ENSMUST00000148736 ENSMUST00000142069 ENSMUST00000134418 ENSMUST00000137878 ENSMUST00000036825 ENSMUST00000134110 ENSMUST00000021071 ENSMUST00000177952 ENSMUST00000108160 ENSMUST00000164945 ENSMUST00000018989 ENSMUST00000103179 ENSMUST00000092802 ENSMUST00000119628 ENSMUST00000134216

Gene Information

Description Not Available

Gene Symbol:   Cog1
Gene Name:   Component of oligomeric golgi complex 1
Aliases:   6030445I15, Ldlb, mKIAA1381
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000018661
Entrez:   16834
UniGene:   Mm.261620
Chromosome Mapping:   11 E2|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.990000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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