PrimePCR™ SYBR® Green Assay: Rac3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0053866

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0045440
Assay Design:   exonic
Chromosome Location:   11:120723507-120723596question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000134660 ENSMUST00000020846 ENSMUST00000144942 ENSMUST00000102600 ENSMUST00000165866 ENSMUST00000092891 ENSMUST00000049020 ENSMUST00000023044 ENSMUST00000067689 ENSMUST00000018156 ENSMUST00000142229 ENSMUST00000043634 ENSMUST00000076060 ENSMUST00000171496 ENSMUST00000010189 ENSMUST00000180430 ENSMUST00000074989 ENSMUST00000018737 ENSMUST00000108858 ENSMUST00000029711 ENSMUST00000107582 ENSMUST00000142819 ENSMUST00000056480 ENSMUST00000163215 ENSMUST00000165762 ENSMUST00000165248 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rac3
Gene Name:   RAS-related C3 botulinum substrate 3
Aliases:   Rac1B
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000018012
Entrez:   170758
UniGene:   Mm.34008
Chromosome Mapping:   11 E2|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.500000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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