PrimePCR™ SYBR® Green Assay: Acvrl1, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0053847

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0045421
Assay Design:   exonic
Chromosome Location:   15:101135305-101135394question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000153102 ENSMUST00000111215 ENSMUST00000075774 ENSMUST00000000542 ENSMUST00000120028 ENSMUST00000117984 ENSMUST00000119063 ENSMUST00000121718 ENSMUST00000124151 ENSMUST00000120754 ENSMUST00000066026 ENSMUST00000004208 ENSMUST00000109075 ENSMUST00000016397 ENSMUST00000033665 ENSMUST00000056355 ENSMUST00000039810 ENSMUST00000074834 ENSMUST00000023226 ENSMUST00000072692 ENSMUST00000169438 ENSMUST00000169714 ENSMUST00000169108 ENSMUST00000076442 ENSMUST00000171562 ENSMUST00000171634 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Acvrl1
Gene Name:   Activin A receptor, type II-like 1
Aliases:   AI115505, AI427544, Acvrlk1, Alk1
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000000530
Entrez:   11482
UniGene:   Mm.279542
Chromosome Mapping:   15|15 F3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.470000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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