PrimePCR™ SYBR® Green Assay: Snrnp200, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0053109

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Snrnp200, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Snrnp200, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0044683
Assay Design:   exonic
Chromosome Location:   2:127230026-127230219question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000103220

Gene Information

On February 19 2002 this locus was switched from human to mouse. The source accession Z70200.1 is almost identical to the mouse BAC clone AC074224 and it matches the mouse cDNA accession BC011390 as well. The human gene is LocusID 23020. [provided by RefSeq Jul 2008]

Gene Symbol:   Snrnp200
Gene Name:   Small nuclear ribonucleoprotein 200 (U5)
Aliases:   A330064G03Rik, Ascc3l1, BC011390, HELIC2, U5-200-KD, U5-200KD
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000003660
Entrez:   320632
Chromosome Mapping:   2 F1|2 62.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download