This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0053109
PrimePCR™ PreAmp for SYBR® Green Assay: Snrnp200, Mouse
PrimePCR™ Template for SYBR® Green Assay: Snrnp200, Mouse
On February 19 2002 this locus was switched from human to mouse. The source accession Z70200.1 is almost identical to the mouse BAC clone AC074224 and it matches the mouse cDNA accession BC011390 as well. The human gene is LocusID 23020. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.