PrimePCR™ SYBR® Green Assay: Fabp6, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0052728

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Fabp6, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Fabp6, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0044302
Assay Design:   exonic
Chromosome Location:   11:43598591-43598721question
Amplicon Length:   101
Splice Variants Targeted:   ENSMUST00000020672

Gene Information

The protein encoded by this gene is part of the fatty acid binding protein family (FABP). FABPs are a family of small highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands and participate in fatty acid uptake transport and metabolism. This protein functions within the ileum the distal 25-30% of the small intestine and plays a role in enterohepatic circulation of bile acids and cholesterol homeostasis. In humans it has been reported that polymorphisms in FABP6 confer a protective effect in obese individuals from developing type 2 diabetes. In mice deficiency of this gene affects bile acid metabolism in a gender-specific manner and was reported to be required for efficient apical to basolateral transport of conjugated bile acids. [provided by RefSeq Jan 2013]

Gene Symbol:   Fabp6
Gene Name:   Fatty acid binding protein 6, ileal (gastrotropin)
Aliases:   I-15P, I-BABP, ILBP, ILBP3, Illbp
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000020405
Entrez:   16204
Chromosome Mapping:   11 B1.1|11 24.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.420000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download