PrimePCR™ SYBR® Green Assay: AC190166.2, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0044074

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0044036
Assay Design:   exonic
Chromosome Location:   Y:13133218-13133322question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000179125 ENSMUST00000177579 ENSMUST00000178559 ENSMUST00000177612 ENSMUST00000177878 ENSMUST00000178669 ENSMUST00000179612 ENSMUST00000179284 ENSMUST00000178050 ENSMUST00000178519 ENSMUST00000178428 ENSMUST00000178689 ENSMUST00000179522 ENSMUST00000179900 ENSMUST00000179219 ENSMUST00000179302 ENSMUST00000039064 ENSMUST00000171795 ENSMUST00000072406 ENSMUST00000164342 ENSMUST00000012440 ENSMUST00000180283 ENSMUST00000107498 ENSMUST00000090942 ENSMUST00000081695 ENSMUST00000109252 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   AC190166.2
Gene Name:   Serine-rich secreted Y-linked protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095769
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 38.050000
Efficiency 83

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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