PrimePCR™ SYBR® Green Assay: Morf4l2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0044024

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Morf4l2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Morf4l2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041808
Assay Design:   exonic
Chromosome Location:   X:136734067-136734263question
Amplicon Length:   167
Splice Variants Targeted:   ENSMUST00000087050 ENSMUST00000113097 ENSMUST00000033797 ENSMUST00000080411 ENSMUST00000155207 ENSMUST00000137605 ENSMUST00000113095 ENSMUST00000166478 ENSMUST00000164609 ENSMUST00000169418 ENSMUST00000166930 ENSMUST00000112588 ENSMUST00000131252 ENSMUST00000113489 ENSMUST00000113487 ENSMUST00000110374 ENSMUST00000099981 ENSMUST00000134720 ENSMUST00000111882 ENSMUST00000111846

Gene Information

Description Not Available

Gene Symbol:   Morf4l2
Gene Name:   Mortality factor 4 like 2
Aliases:   2410017O14Rik, Mrgx, Sid393p, mKIAA0026
RefSeq:   NC_000086.7 NT_039716.8
Ensembl:   ENSMUSG00000031422
Entrez:   56397
Chromosome Mapping:   X F1|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 38.660000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download