PrimePCR™ SYBR® Green Assay: Vmn1r100, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043970

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Vmn1r100, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Vmn1r100, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041754
Assay Design:   exonic
Chromosome Location:   7:20418554-20418655question
Amplicon Length:   72
Splice Variants Targeted:   ENSMUST00000165330 ENSMUST00000079099 ENSMUST00000098747 ENSMUST00000164526 ENSMUST00000168984 ENSMUST00000169774 ENSMUST00000172989 ENSMUST00000177815 ENSMUST00000164245 ENSMUST00000098744 ENSMUST00000169689 ENSMUST00000173886 ENSMUST00000174364 ENSMUST00000179079 ENSMUST00000174538 ENSMUST00000173723 ENSMUST00000168794 ENSMUST00000098737 ENSMUST00000169374 ENSMUST00000164288 ENSMUST00000166937 ENSMUST00000164683 ENSMUST00000105197 ENSMUST00000164409 ENSMUST00000166948 ENSMUST00000105200 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Vmn1r100
Gene Name:   Vomeronasal 1 receptor 100
Aliases:   ENSMUSG00000074331, Gm10671
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000096386
Entrez:   100043536
Chromosome Mapping:   7 A3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.770000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download