PrimePCR™ SYBR® Green Assay: AC116421.1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043964

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AC116421.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AC116421.1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041748
Assay Design:   exonic
Chromosome Location:   9:84423961-84424073question
Amplicon Length:   83
Splice Variants Targeted:   ENSMUST00000096427 ENSMUST00000027777 ENSMUST00000048655 ENSMUST00000040021 ENSMUST00000033952 ENSMUST00000115245 ENSMUST00000054212 ENSMUST00000178075 ENSMUST00000085044 ENSMUST00000166103 ENSMUST00000006854 ENSMUST00000177716 ENSMUST00000102898 ENSMUST00000138587 ENSMUST00000020732 ENSMUST00000047441 ENSMUST00000081658 ENSMUST00000052872 ENSMUST00000135230 ENSMUST00000048774 ENSMUST00000166192 ENSMUST00000171235 ENSMUST00000095087 ENSMUST00000091146 ENSMUST00000102872 ENSMUST00000147488 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   AC116421.1
Gene Name:   60S ribosomal protein L7a
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000096319
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 37.220000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download