PrimePCR™ SYBR® Green Assay: AC147264.1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043935

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AC147264.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AC147264.1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041719
Assay Design:   exonic
Chromosome Location:   Y:79149639-79149772question
Amplicon Length:   104
Splice Variants Targeted:   ENSMUST00000177666 ENSMUST00000179829 ENSMUST00000178181 ENSMUST00000179949 ENSMUST00000178149 ENSMUST00000179922 ENSMUST00000178903 ENSMUST00000178326 ENSMUST00000181549 ENSMUST00000178889 ENSMUST00000115496 ENSMUST00000171947 ENSMUST00000100105 ENSMUST00000181205 ENSMUST00000181163 ENSMUST00000179070 ENSMUST00000181357

Gene Information

Description Not Available

Gene Symbol:   AC147264.1
Gene Name:   Uncharacterized protein LOC100042428
Aliases:   Not Available
RefSeq:   NC_000087.7 NT_187050.1
Ensembl:   ENSMUSG00000095867
Entrez:   100042428
Chromosome Mapping:   Y|Y

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.510000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download