PrimePCR™ SYBR® Green Assay: Gm9125, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043930

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gm9125, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gm9125, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041714
Assay Design:   exonic
Chromosome Location:   3:93938165-93938306question
Amplicon Length:   112
Splice Variants Targeted:   ENSMUST00000179064 ENSMUST00000178358 ENSMUST00000181541 ENSMUST00000094413 ENSMUST00000179087 ENSMUST00000090853 ENSMUST00000075953 ENSMUST00000081780 ENSMUST00000098881 ENSMUST00000177735 ENSMUST00000098879 ENSMUST00000180746 ENSMUST00000178458 ENSMUST00000091692 ENSMUST00000161475 ENSMUST00000167916 ENSMUST00000159524 ENSMUST00000159517 ENSMUST00000098878

Gene Information

Description Not Available

Gene Symbol:   Gm9125
Gene Name:   Predicted gene 9125
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095822
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 37.490000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download