PrimePCR™ SYBR® Green Assay: Tcstv3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043929

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Tcstv3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tcstv3, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041713
Assay Design:   exonic
Chromosome Location:   13:120317817-120317962question
Amplicon Length:   116
Splice Variants Targeted:   ENSMUST00000179455 ENSMUST00000179131 ENSMUST00000178510 ENSMUST00000179502 ENSMUST00000180768 ENSMUST00000080505 ENSMUST00000179071 ENSMUST00000178828 ENSMUST00000177561 ENSMUST00000079524 ENSMUST00000181748 ENSMUST00000179905 ENSMUST00000081558 ENSMUST00000178349 ENSMUST00000082321 ENSMUST00000171561 ENSMUST00000026168

Gene Information

Description Not Available

Gene Symbol:   Tcstv3
Gene Name:   2-cell-stage, variable group, member 3
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095821
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 38.380000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download