PrimePCR™ SYBR® Green Assay: CT030687.1, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043925

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041709
Assay Design:   exonic
Chromosome Location:   12:88249911-88250051question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000078079 ENSMUST00000168382 ENSMUST00000110152 ENSMUST00000164838 ENSMUST00000164517 ENSMUST00000181313 ENSMUST00000110149 ENSMUST00000110147 ENSMUST00000095510 ENSMUST00000180053 ENSMUST00000110148 ENSMUST00000180285 ENSMUST00000178301 ENSMUST00000178852 ENSMUST00000177720 ENSMUST00000179468 ENSMUST00000101168 ENSMUST00000177747 ENSMUST00000091715

Gene Information

Description Not Available

Gene Symbol:   CT030687.1
Gene Name:   Eukaryotic translation initiation factor 1A-like 2
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000095799
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 38.290000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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