PrimePCR™ SYBR® Green Assay: AL808139.2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043902

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AL808139.2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AL808139.2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041686
Assay Design:   exonic
Chromosome Location:   X:37380910-37381101question
Amplicon Length:   162
Splice Variants Targeted:   ENSMUST00000028080 ENSMUST00000085555 ENSMUST00000075922 ENSMUST00000002084 ENSMUST00000114461 ENSMUST00000178486 ENSMUST00000023741 ENSMUST00000020366 ENSMUST00000115203 ENSMUST00000096459 ENSMUST00000115188 ENSMUST00000123851 ENSMUST00000115189 ENSMUST00000115198 ENSMUST00000081327 ENSMUST00000115172 ENSMUST00000096457 ENSMUST00000033965 ENSMUST00000129821 ENSMUST00000103111 ENSMUST00000103112 ENSMUST00000109800 ENSMUST00000037580 ENSMUST00000114176 ENSMUST00000114172 ENSMUST00000171268 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   AL808139.2
Gene Name:   Reproductive homeobox 3A
Aliases:   Not Available
RefSeq:   NC_000086.7 NT_187037.1
Ensembl:   ENSMUSG00000095601
Entrez:   100861623
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.996700
y-intercept 37.270000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download