PrimePCR™ SYBR® Green Assay: Zfp955a, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043832

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Zfp955a, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Zfp955a, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041616
Assay Design:   exonic
Chromosome Location:   17:33244038-33255023question
Amplicon Length:   70
Splice Variants Targeted:   ENSMUST00000131237 ENSMUST00000136516 ENSMUST00000132848 ENSMUST00000109997 ENSMUST00000105315 ENSMUST00000099439 ENSMUST00000107989 ENSMUST00000076195 ENSMUST00000101547 ENSMUST00000091563 ENSMUST00000167516 ENSMUST00000073201 ENSMUST00000087666 ENSMUST00000157017 ENSMUST00000008830 ENSMUST00000099414 ENSMUST00000054072 ENSMUST00000169125 ENSMUST00000130458 ENSMUST00000154063 ENSMUST00000149782 ENSMUST00000126915 ENSMUST00000089906

Gene Information

Description Not Available

Gene Symbol:   Zfp955a
Gene Name:   Zinc finger protein 955A
Aliases:   AI842447, Zfp883a
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000094441
Entrez:   77652
Chromosome Mapping:   17 B1|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 39.590000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download