PrimePCR™ SYBR® Green Assay: Vmn2r96, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043746

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Vmn2r96, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Vmn2r96, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041530
Assay Design:   exonic
Chromosome Location:   17:18597285-18597392question
Amplicon Length:   78
Splice Variants Targeted:   ENSMUST00000049810 ENSMUST00000057037 ENSMUST00000165029 ENSMUST00000169130 ENSMUST00000163803 ENSMUST00000173815 ENSMUST00000166914 ENSMUST00000172359 ENSMUST00000169686 ENSMUST00000172190 ENSMUST00000166327 ENSMUST00000177244 ENSMUST00000165692 ENSMUST00000170424 ENSMUST00000176107 ENSMUST00000166081 ENSMUST00000172203 ENSMUST00000168050 ENSMUST00000167382 ENSMUST00000167464 ENSMUST00000042090 ENSMUST00000167314 ENSMUST00000169559

Gene Information

Description Not Available

Gene Symbol:   Vmn2r96
Gene Name:   Vomeronasal 2, receptor 96
Aliases:   EG433070
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000091679
Entrez:   433070
Chromosome Mapping:   17 A3.2|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 38.860000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download