PrimePCR™ SYBR® Green Assay: Vmn1r113, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qMmuCEP0043743

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Vmn1r113, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Vmn1r113, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041527
Assay Design:   exonic
Chromosome Location:   7:20787958-20788108question
Amplicon Length:   121
Splice Variants Targeted:   ENSMUST00000079099 ENSMUST00000169774 ENSMUST00000172989 ENSMUST00000164245 ENSMUST00000163461 ENSMUST00000173886 ENSMUST00000173723 ENSMUST00000168794 ENSMUST00000166937 ENSMUST00000164683 ENSMUST00000164409 ENSMUST00000166948 ENSMUST00000165961 ENSMUST00000105195 ENSMUST00000173484 ENSMUST00000163096 ENSMUST00000172551 ENSMUST00000165916 ENSMUST00000166079 ENSMUST00000174637 ENSMUST00000174643 ENSMUST00000167871 ENSMUST00000171745 ENSMUST00000168392

Gene Information

Description Not Available

Gene Symbol:   Vmn1r113
Gene Name:   Vomeronasal 1 receptor 113
Aliases:   EG436135, Gm5748
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000091638
Entrez:   436135
Chromosome Mapping:   7 A3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 38.760000
Efficiency 86

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download