PrimePCR™ SYBR® Green Assay: Speer4e, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qMmuCEP0043728

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041512
Assay Design:   exonic
Chromosome Location:   5:14934946-14935063question
Amplicon Length:   88
Splice Variants Targeted:   ENSMUST00000162387 ENSMUST00000178487 ENSMUST00000168875 ENSMUST00000170224 ENSMUST00000072286 ENSMUST00000079447 ENSMUST00000095004 ENSMUST00000094946 ENSMUST00000063524 ENSMUST00000088236 ENSMUST00000074148 ENSMUST00000053257 ENSMUST00000155721 ENSMUST00000166086 ENSMUST00000076099 ENSMUST00000035980 ENSMUST00000159973 ENSMUST00000096953 ENSMUST00000167908 ENSMUST00000095006 ENSMUST00000119100 ENSMUST00000092696 ENSMUST00000095005 ENSMUST00000180847 ENSMUST00000180994 ENSMUST00000181616 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Speer4e
Gene Name:   Spermatogenesis associated glutamate (E)-rich protein 4e
Aliases:   Not Available
RefSeq:   NC_000071.6 NT_039299.7
Ensembl:   ENSMUSG00000091255
Entrez:   624245
UniGene:   Not Available
Chromosome Mapping:   5 A1|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 39.810000
Efficiency 86

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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