PrimePCR™ SYBR® Green Assay: Zscan4d, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043718

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Zscan4d, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Zscan4d, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041502
Assay Design:   exonic
Chromosome Location:   7:11165260-11165365question
Amplicon Length:   76
Splice Variants Targeted:   ENSMUST00000168158 ENSMUST00000131379 ENSMUST00000067210 ENSMUST00000165848 ENSMUST00000094850 ENSMUST00000179596 ENSMUST00000166753 ENSMUST00000145237 ENSMUST00000141491 ENSMUST00000091440 ENSMUST00000095541 ENSMUST00000088547 ENSMUST00000180213 ENSMUST00000023611 ENSMUST00000057576 ENSMUST00000042561 ENSMUST00000169547 ENSMUST00000087703 ENSMUST00000165912 ENSMUST00000065916 ENSMUST00000028633 ENSMUST00000103234 ENSMUST00000044642 ENSMUST00000170773 ENSMUST00000068261

Gene Information

Description Not Available

Gene Symbol:   Zscan4d
Gene Name:   Zinc finger and SCAN domain containing 4D
Aliases:   EG545913
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000090714
Entrez:   545913
Chromosome Mapping:   7 A1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 37.210000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download