PrimePCR™ SYBR® Green Assay: Cfhr3, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043716

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041500
Assay Design:   exonic
Chromosome Location:   1:139593803-139593933question
Amplicon Length:   101
Splice Variants Targeted:   ENSMUST00000023965 ENSMUST00000160423 ENSMUST00000170441 ENSMUST00000027612 ENSMUST00000111989 ENSMUST00000111986 ENSMUST00000094489 ENSMUST00000066859 ENSMUST00000111977 ENSMUST00000111976 ENSMUST00000163116 ENSMUST00000094203 ENSMUST00000121484 ENSMUST00000108831

Gene Information

Description Not Available

Gene Symbol:   Cfhr3
Gene Name:   Complement factor H-related 3
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000090623
UniGene:   Mm.483958
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 41.440000
Efficiency 80

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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