PrimePCR™ SYBR® Green Assay: Uba52, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043702

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Uba52, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Uba52, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041486
Assay Design:   exonic
Chromosome Location:   8:70508433-70509312question
Amplicon Length:   82
Splice Variants Targeted:   ENSMUST00000089430 ENSMUST00000071965 ENSMUST00000051014 ENSMUST00000081864 ENSMUST00000110272 ENSMUST00000120942 ENSMUST00000072061 ENSMUST00000021662 ENSMUST00000082002 ENSMUST00000060603 ENSMUST00000107376 ENSMUST00000093901 ENSMUST00000026442 ENSMUST00000106225 ENSMUST00000140679 ENSMUST00000129909 ENSMUST00000125184 ENSMUST00000081940 ENSMUST00000135446 ENSMUST00000124967 ENSMUST00000165126 ENSMUST00000121623

Gene Information

Description Not Available

Gene Symbol:   Uba52
Gene Name:   Ubiquitin A-52 residue ribosomal protein fusion product 1
Aliases:   D8Ertd21e, Gm1863, Rps27a, Ubb, Ubc
RefSeq:   NC_000074.6 NT_039462.8
Ensembl:   ENSMUSG00000090137
Entrez:   22186
Chromosome Mapping:   8 B3.3|8 33.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 37.320000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download