PrimePCR™ SYBR® Green Assay: Phyhd1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043665

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Phyhd1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Phyhd1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041449
Assay Design:   exonic
Chromosome Location:   2:30269689-30269789question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000075452 ENSMUST00000034761 ENSMUST00000034230 ENSMUST00000063414 ENSMUST00000179310 ENSMUST00000100209 ENSMUST00000171444 ENSMUST00000171949 ENSMUST00000004780 ENSMUST00000109411 ENSMUST00000018094 ENSMUST00000031273 ENSMUST00000118280 ENSMUST00000062092 ENSMUST00000154647 ENSMUST00000113645 ENSMUST00000133877 ENSMUST00000139719 ENSMUST00000113643 ENSMUST00000150695 ENSMUST00000091132 ENSMUST00000071782 ENSMUST00000065987 ENSMUST00000115839

Gene Information

Description Not Available

Gene Symbol:   Phyhd1
Gene Name:   Phytanoyl-CoA dioxygenase domain containing 1
Aliases:   Not Available
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000079484
Entrez:   227696
Chromosome Mapping:   2 B|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 37.980000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download