PrimePCR™ SYBR® Green Assay: Ccdc24, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043632

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Ccdc24, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Ccdc24, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041416
Assay Design:   exonic
Chromosome Location:   4:117872048-117872140question
Amplicon Length:   63
Splice Variants Targeted:   ENSMUST00000040689 ENSMUST00000139848 ENSMUST00000065601 ENSMUST00000111936 ENSMUST00000111899 ENSMUST00000099392 ENSMUST00000062322 ENSMUST00000073029 ENSMUST00000161977 ENSMUST00000163091 ENSMUST00000022356 ENSMUST00000035340 ENSMUST00000074660 ENSMUST00000062483 ENSMUST00000060930 ENSMUST00000066026 ENSMUST00000164853 ENSMUST00000106422 ENSMUST00000171052 ENSMUST00000166325 ENSMUST00000040835 ENSMUST00000061260 ENSMUST00000099092 ENSMUST00000023504 ENSMUST00000008748 ENSMUST00000140466 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Ccdc24
Gene Name:   Coiled-coil domain containing 24
Aliases:   Gm1028
RefSeq:   NC_000070.6 NT_039264.7
Ensembl:   ENSMUSG00000078588
Entrez:   381546
Chromosome Mapping:   4 D2.1|4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 37.230000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download