PrimePCR™ SYBR® Green Assay: Hist2h3b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043572

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hist2h3b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hist2h3b, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041356
Assay Design:   exonic
Chromosome Location:   3:96268855-96269029question
Amplicon Length:   145
Splice Variants Targeted:   ENSMUST00000141137 ENSMUST00000058440 ENSMUST00000003038 ENSMUST00000091754 ENSMUST00000099704 ENSMUST00000080859 ENSMUST00000075558 ENSMUST00000105107 ENSMUST00000105105 ENSMUST00000091752 ENSMUST00000091703 ENSMUST00000091701 ENSMUST00000170800 ENSMUST00000027985 ENSMUST00000179893 ENSMUST00000032813 ENSMUST00000116614 ENSMUST00000163718 ENSMUST00000029017 ENSMUST00000115467 ENSMUST00000154266 ENSMUST00000076654 ENSMUST00000112377 ENSMUST00000033652 ENSMUST00000112380 ENSMUST00000084820 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Hist2h3b
Gene Name:   Histone cluster 2, H3b
Aliases:   H3-615, H3-616
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000074403
Entrez:   319154
Chromosome Mapping:   3 F2.1|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.996100
y-intercept 42.880000
Efficiency 81

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download