PrimePCR™ SYBR® Green Assay: Amy2a1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043480

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041263
Assay Design:   exonic
Chromosome Location:   3:113529478-113529597question
Amplicon Length:   90
Splice Variants Targeted:   ENSMUST00000154873 ENSMUST00000114799 ENSMUST00000156862 ENSMUST00000080572 ENSMUST00000019363 ENSMUST00000094753 ENSMUST00000117859 ENSMUST00000064493 ENSMUST00000082094 ENSMUST00000036606 ENSMUST00000028279 ENSMUST00000107042 ENSMUST00000049053 ENSMUST00000098673 ENSMUST00000179568 ENSMUST00000179314 ENSMUST00000098667 ENSMUST00000132353 ENSMUST00000067980 ENSMUST00000106540 ENSMUST00000142505 ENSMUST00000048002

Gene Information

Description Not Available

Gene Symbol:   Amy2a1
Gene Name:   Amylase 2a1
Aliases:   Amy2-1, OTTMUSG00000022432, mAmy2-6
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000070360
Entrez:   100043207
UniGene:   Not Available
Chromosome Mapping:   3 F3|3 50.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 37.960000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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