PrimePCR™ SYBR® Green Assay: Krtap9-1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043479

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Krtap9-1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Krtap9-1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041262
Assay Design:   exonic
Chromosome Location:   11:99873826-99873949question
Amplicon Length:   94
Splice Variants Targeted:   ENSMUST00000082104 ENSMUST00000109570 ENSMUST00000029158 ENSMUST00000098179 ENSMUST00000027494 ENSMUST00000041819 ENSMUST00000052629 ENSMUST00000111235 ENSMUST00000097462 ENSMUST00000141548 ENSMUST00000088263 ENSMUST00000034400 ENSMUST00000106619 ENSMUST00000159786 ENSMUST00000113547 ENSMUST00000118544 ENSMUST00000078748 ENSMUST00000131996 ENSMUST00000064225 ENSMUST00000108044 ENSMUST00000065103 ENSMUST00000015901 ENSMUST00000039484 ENSMUST00000111086 ENSMUST00000122965 ENSMUST00000079390 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Krtap9-1
Gene Name:   Keratin associated protein 9-1
Aliases:   Not Available
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000070335
Entrez:   16705
Chromosome Mapping:   11 D|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 37.030000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download