PrimePCR™ SYBR® Green Assay: Vmn1r19, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043445

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Vmn1r19, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Vmn1r19, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041228
Assay Design:   exonic
Chromosome Location:   6:57404743-57404919question
Amplicon Length:   147
Splice Variants Targeted:   ENSMUST00000079669 ENSMUST00000176252 ENSMUST00000078186 ENSMUST00000171186 ENSMUST00000176073 ENSMUST00000176954 ENSMUST00000177267 ENSMUST00000176395 ENSMUST00000164732 ENSMUST00000089830 ENSMUST00000176085 ENSMUST00000081186 ENSMUST00000095862 ENSMUST00000176874 ENSMUST00000168416 ENSMUST00000078890 ENSMUST00000176177 ENSMUST00000079584

Gene Information

Description Not Available

Gene Symbol:   Vmn1r19
Gene Name:   Vomeronasal 1 receptor 19
Aliases:   V1rc27
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000068426
Entrez:   171200
Chromosome Mapping:   6 B3|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.300000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download