PrimePCR™ SYBR® Green Assay: Mageb1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043347

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Mageb1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Mageb1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041130
Assay Design:   exonic
Chromosome Location:   X:92015470-92015572question
Amplicon Length:   73
Splice Variants Targeted:   ENSMUST00000114013 ENSMUST00000145419 ENSMUST00000041782 ENSMUST00000167020 ENSMUST00000092112 ENSMUST00000099471 ENSMUST00000072269 ENSMUST00000063607 ENSMUST00000126792 ENSMUST00000106549 ENSMUST00000106550 ENSMUST00000168529 ENSMUST00000174724 ENSMUST00000173126 ENSMUST00000100750 ENSMUST00000170481 ENSMUST00000154132 ENSMUST00000104936 ENSMUST00000101397 ENSMUST00000063726 ENSMUST00000101396

Gene Information

Description Not Available

Gene Symbol:   Mageb1
Gene Name:   Melanoma antigen, family B, 1
Aliases:   MAGE-Xp, Mage-b1, Mage-rs1, Magel1, Smage1, dam1
RefSeq:   NC_000086.7 NT_039706.8
Ensembl:   ENSMUSG00000062162
Entrez:   17145
Chromosome Mapping:   X C2|X 35.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 37.360000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download