PrimePCR™ SYBR® Green Assay: Hist1h2af, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043343

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hist1h2af, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hist1h2af, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041126
Assay Design:   exonic
Chromosome Location:   13:23534167-21806416question
Amplicon Length:   112
Splice Variants Targeted:   ENSMUST00000001147 ENSMUST00000070124 ENSMUST00000074752 ENSMUST00000091751 ENSMUST00000091745 ENSMUST00000081342 ENSMUST00000091742 ENSMUST00000091741 ENSMUST00000073261 ENSMUST00000102969 ENSMUST00000090776 ENSMUST00000171127 ENSMUST00000078369 ENSMUST00000058593 ENSMUST00000096494 ENSMUST00000072391 ENSMUST00000120452 ENSMUST00000070070 ENSMUST00000074556 ENSMUST00000121799 ENSMUST00000091708 ENSMUST00000028239 ENSMUST00000047697 ENSMUST00000108817 ENSMUST00000090782 ENSMUST00000078756 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Hist1h2af
Gene Name:   Histone cluster 1, H2af
Aliases:   H2a-221
RefSeq:   NC_000079.6 NT_039578.8
Ensembl:   ENSMUSG00000061991
Entrez:   319173
Chromosome Mapping:   13 A3.1|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.080000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download