PrimePCR™ SYBR® Green Assay: Ankrd24, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043222

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0041005
Assay Design:   exonic
Chromosome Location:   10:81629628-81629732question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000123896 ENSMUST00000153573 ENSMUST00000119336 ENSMUST00000129622 ENSMUST00000045068 ENSMUST00000059286 ENSMUST00000089023 ENSMUST00000089022 ENSMUST00000113024 ENSMUST00000053969 ENSMUST00000166429 ENSMUST00000027233 ENSMUST00000043464 ENSMUST00000000291 ENSMUST00000089581 ENSMUST00000066582 ENSMUST00000107162 ENSMUST00000107166 ENSMUST00000107165 ENSMUST00000103107 ENSMUST00000130916 ENSMUST00000100539 ENSMUST00000085786 ENSMUST00000164148 ENSMUST00000095451

Gene Information

Description Not Available

Gene Symbol:   Ankrd24
Gene Name:   Ankyrin repeat domain 24
Aliases:   4631433D01Rik, 5730519E19Rik, D10Bur2e
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000054708
Entrez:   70615
UniGene:   Mm.304382
Chromosome Mapping:   10 C1|10 43.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 37.440000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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