PrimePCR™ SYBR® Green Assay: Sprr2b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043190

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Sprr2b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Sprr2b, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040973
Assay Design:   exonic
Chromosome Location:   3:92317668-92317817question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000160770 ENSMUST00000013220 ENSMUST00000048112 ENSMUST00000057209 ENSMUST00000112325 ENSMUST00000154248 ENSMUST00000074449 ENSMUST00000090871 ENSMUST00000071805 ENSMUST00000061038 ENSMUST00000043151 ENSMUST00000112470 ENSMUST00000156172 ENSMUST00000035836 ENSMUST00000165052 ENSMUST00000113532

Gene Information

Description Not Available

Gene Symbol:   Sprr2b
Gene Name:   Small proline-rich protein 2B
Aliases:   Not Available
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000050092
Entrez:   20756
Chromosome Mapping:   3 F1|3 45.2 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.100000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download