PrimePCR™ SYBR® Green Assay: Mfap1b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qMmuCEP0043180

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Mfap1b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Mfap1b, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040963
Assay Design:   exonic
Chromosome Location:   2:121469861-121469999question
Amplicon Length:   109
Splice Variants Targeted:   ENSMUST00000066509 ENSMUST00000114608 ENSMUST00000114627 ENSMUST00000094787 ENSMUST00000128646 ENSMUST00000149235 ENSMUST00000066928 ENSMUST00000148891 ENSMUST00000131942 ENSMUST00000110161 ENSMUST00000041197 ENSMUST00000180359 ENSMUST00000179669 ENSMUST00000142443 ENSMUST00000165379 ENSMUST00000141911 ENSMUST00000170618 ENSMUST00000096241 ENSMUST00000038753 ENSMUST00000107586 ENSMUST00000056732 ENSMUST00000089926 ENSMUST00000084241 ENSMUST00000105912 ENSMUST00000047075 ENSMUST00000047157 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Mfap1b
Gene Name:   Microfibrillar-associated protein 1B
Aliases:   Not Available
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000048222
Entrez:   100034361
Chromosome Mapping:   2 F1|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.550000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download