PrimePCR™ SYBR® Green Assay: Hmga1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043172

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hmga1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hmga1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040955
Assay Design:   exonic
Chromosome Location:   17:27562773-27562910question
Amplicon Length:   108
Splice Variants Targeted:   ENSMUST00000117600 ENSMUST00000114888 ENSMUST00000119486 ENSMUST00000117254 ENSMUST00000118570 ENSMUST00000079869 ENSMUST00000105046 ENSMUST00000070174 ENSMUST00000174697 ENSMUST00000174072 ENSMUST00000056660 ENSMUST00000119810 ENSMUST00000032968 ENSMUST00000161028 ENSMUST00000089362 ENSMUST00000089360

Gene Information

This locus encodes a member of the nuclear non-histone high mobility group protein family. This architectural transcription factor binds to A-T rich DNA sequences and participates in enhanceosome formation chromatin remodeling and regulation of transcription. This protein functions in many cellular processes including cell growth and differentiation. Alternatively spliced transcript variants have been described. [provided by RefSeq Oct 2009]

Gene Symbol:   Hmga1
Gene Name:   High mobility group AT-hook 1
Aliases:   AL023995, Hmga1a, Hmga1b, Hmgi, Hmgiy, Hmgy
RefSeq:   NC_000083.6 NT_039649.8 NT_187005.1
Ensembl:   ENSMUSG00000046711
Entrez:   15361
Chromosome Mapping:   17 A3.3|17 13.19 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.770000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download