PrimePCR™ SYBR® Green Assay: Foxn3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043109

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Foxn3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Foxn3, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040892
Assay Design:   exonic
Chromosome Location:   12:99388441-99388535question
Amplicon Length:   65
Splice Variants Targeted:   ENSMUST00000060397 ENSMUST00000166696 ENSMUST00000095388 ENSMUST00000108176 ENSMUST00000108175 ENSMUST00000046177 ENSMUST00000101030 ENSMUST00000046859 ENSMUST00000177451 ENSMUST00000177269 ENSMUST00000176928 ENSMUST00000085108 ENSMUST00000000896

Gene Information

Description Not Available

Gene Symbol:   Foxn3
Gene Name:   Forkhead box N3
Aliases:   5430426H20Rik, AA589593, AW556347, Ches1, Ches1l, HTLFL1
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000033713
Entrez:   71375
Chromosome Mapping:   12 E|12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 38.130000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download