PrimePCR™ SYBR® Green Assay: Tomm6, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043106

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Tomm6, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tomm6, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040889
Assay Design:   exonic
Chromosome Location:   17:47686787-47686943question
Amplicon Length:   127
Splice Variants Targeted:   ENSMUST00000034713 ENSMUST00000170430 ENSMUST00000161241 ENSMUST00000159207 ENSMUST00000160486 ENSMUST00000167546 ENSMUST00000126879 ENSMUST00000056470 ENSMUST00000085668 ENSMUST00000042312 ENSMUST00000120784 ENSMUST00000032719 ENSMUST00000161582 ENSMUST00000060601 ENSMUST00000069926 ENSMUST00000114679 ENSMUST00000133983 ENSMUST00000060072 ENSMUST00000146730 ENSMUST00000155016 ENSMUST00000041124 ENSMUST00000153424 ENSMUST00000065698 ENSMUST00000107624 ENSMUST00000107623 ENSMUST00000062652 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Tomm6
Gene Name:   Translocase of outer mitochondrial membrane 6 homolog (yeast)
Aliases:   1110002E23Rik, AI663970
RefSeq:   NC_000083.6 NT_039649.8
Ensembl:   ENSMUSG00000033475
Entrez:   66119
Chromosome Mapping:   17 C|17

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.480000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download