PrimePCR™ SYBR® Green Assay: Rbm10, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043094

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Rbm10, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Rbm10, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040877
Assay Design:   exonic
Chromosome Location:   X:20647346-20647481question
Amplicon Length:   106
Splice Variants Targeted:   ENSMUST00000087996 ENSMUST00000061878 ENSMUST00000068878 ENSMUST00000004827 ENSMUST00000111277 ENSMUST00000115375 ENSMUST00000115374 ENSMUST00000084383 ENSMUST00000082089 ENSMUST00000177738 ENSMUST00000064911 ENSMUST00000046333 ENSMUST00000109896 ENSMUST00000112685 ENSMUST00000026296 ENSMUST00000112683 ENSMUST00000026295 ENSMUST00000094456 ENSMUST00000028951 ENSMUST00000109875 ENSMUST00000028351 ENSMUST00000022137 ENSMUST00000168772 ENSMUST00000114082 ENSMUST00000091252 ENSMUST00000027290 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rbm10
Gene Name:   RNA binding motif protein 10
Aliases:   E430039K10Rik
RefSeq:   NC_000086.7 NT_039700.8
Ensembl:   ENSMUSG00000031060
Entrez:   236732
Chromosome Mapping:   X A1.3|X

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.080000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download