PrimePCR™ SYBR® Green Assay: Fam89b, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043048

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040831
Assay Design:   exonic
Chromosome Location:   19:5728507-5728651question
Amplicon Length:   115
Splice Variants Targeted:   ENSMUST00000031376 ENSMUST00000027455 ENSMUST00000064924 ENSMUST00000001460 ENSMUST00000167201 ENSMUST00000168445 ENSMUST00000116558 ENSMUST00000099955 ENSMUST00000161368

Gene Information

Description Not Available

Gene Symbol:   Fam89b
Gene Name:   Family with sequence similarity 89, member B
Aliases:   1110021A21Rik, MMTV, Mtvr2
RefSeq:   NC_000085.6 NT_082868.6
Ensembl:   ENSMUSG00000024939
Entrez:   17826
UniGene:   Mm.391142
Chromosome Mapping:   19 A|19 2.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.940000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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