PrimePCR™ SYBR® Green Assay: Rplp1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0043010

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040793
Assay Design:   exonic
Chromosome Location:   9:61913333-61913451question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000005600 ENSMUST00000076961 ENSMUST00000169266 ENSMUST00000163320 ENSMUST00000005578 ENSMUST00000073722 ENSMUST00000164001 ENSMUST00000174699 ENSMUST00000057195 ENSMUST00000107891 ENSMUST00000117282 ENSMUST00000085383 ENSMUST00000020655 ENSMUST00000109090 ENSMUST00000109091 ENSMUST00000037900 ENSMUST00000106557 ENSMUST00000038650 ENSMUST00000027690 ENSMUST00000109816 ENSMUST00000028969 ENSMUST00000164120 ENSMUST00000178997 ENSMUST00000121646 ENSMUST00000121325 ENSMUST00000121766 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rplp1
Gene Name:   Ribosomal protein, large, P1
Aliases:   2410042H16Rik, Arpp1, C430017H15Rik
RefSeq:   NC_000075.6 NT_039474.8
Ensembl:   ENSMUSG00000007892
Entrez:   56040
UniGene:   Mm.3158
Chromosome Mapping:   9 B|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 38.100000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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