PrimePCR™ SYBR® Green Assay: Eif4e1b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0042986

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Eif4e1b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Eif4e1b, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040769
Assay Design:   exonic
Chromosome Location:   13:54784355-54784470question
Amplicon Length:   86
Splice Variants Targeted:   ENSMUST00000142158 ENSMUST00000139184 ENSMUST00000132005 ENSMUST00000126785 ENSMUST00000132728 ENSMUST00000110003 ENSMUST00000152204

Gene Information

Description Not Available

Gene Symbol:   Eif4e1b
Gene Name:   Eukaryotic translation initiation factor 4E family member 1B
Aliases:   AA473955, Eif4eloo, Gm273
RefSeq:   NC_000079.6 NT_039589.8
Ensembl:   ENSMUSG00000074895
Entrez:   218268
Chromosome Mapping:   13 B1|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 41.800000
Efficiency 80

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download