PrimePCR™ SYBR® Green Assay: Rpl13a, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040629
Assay Design:   exonic
Chromosome Location:   7:45125911-45126010question
Amplicon Length:   70
Splice Variants Targeted:   ENSMUST00000150350 ENSMUST00000124300

Gene Information

Description Not Available

Gene Symbol:   Rpl13a
Gene Name:   Ribosomal protein L13A
Aliases:   1810026N22Rik, Tstap198-7, tum-antigen
RefSeq:   NC_000073.6 NT_187035.1
Ensembl:   ENSMUSG00000074129
Entrez:   22121
UniGene:   Mm.180458
Chromosome Mapping   7 B4|7 69.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 38.160000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev C Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev D Click to download
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