PrimePCR™ SYBR® Green Assay: Agbl5, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0042525

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040307
Assay Design:   exonic
Chromosome Location:   5:30891063-30891188question
Amplicon Length:   96
Splice Variants Targeted:   ENSMUST00000128744 ENSMUST00000069705 ENSMUST00000031057 ENSMUST00000046182 ENSMUST00000114704 ENSMUST00000061213 ENSMUST00000114696 ENSMUST00000114700 ENSMUST00000124908

Gene Information

Description Not Available

Gene Symbol:   Agbl5
Gene Name:   ATP/GTP binding protein-like 5
Aliases:   4930455N08, 9430057O19Rik, CCP5
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000029165
Entrez:   231093
UniGene:   Mm.440364
Chromosome Mapping:   5 B1|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.160000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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