PrimePCR™ SYBR® Green Assay: Lmna, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0042513

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040295
Assay Design:   exonic
Chromosome Location:   3:88484619-88484765question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000029699 ENSMUST00000120377 ENSMUST00000036252

Gene Information

Description Not Available

Gene Symbol:   Lmna
Gene Name:   Lamin A
Aliases:   Dhe
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000028063
Entrez:   16905
UniGene:   Mm.243014
Chromosome Mapping:   3 F1|3 42.6 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.670000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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