PrimePCR™ SYBR® Green Assay: Cldn10, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0042468

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040250
Assay Design:   exonic
Chromosome Location:   14:118874208-118874352question
Amplicon Length:   115
Splice Variants Targeted:   ENSMUST00000100314 ENSMUST00000123747 ENSMUST00000047761 ENSMUST00000071546

Gene Information

This intronless gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight unction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets and also play critical roles in maintaining cell polarity and signal transductions. Six alternatively spliced transcript variants have been identified which encode different isoforms with distinct electric charge of the first extracellular loop and with or without the fourth transmembrane region. These isoforms exhibit distinct localization and function in paracellular anion or cation permeability. [provided by RefSeq Aug 2010]

Gene Symbol:   Cldn10
Gene Name:   Claudin 10
Aliases:   6720456I16Rik, Cldn10a, Cldn10b, D14Ertd728e
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000022132
Entrez:   58187
UniGene:   Mm.390755
Chromosome Mapping:   14 E4|14 60.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.930000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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