PrimePCR™ SYBR® Green Assay: Klra10, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Klra10, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Klra10, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0005082
Assay Design:   Exonic
Chromosome Location:   6:130279269-130279436question
Amplicon Length:   138
Splice Variants Targeted:   ENSMUST00000040821 ENSMUST00000130053 ENSMUST00000122985 ENSMUST00000127380 ENSMUST00000139581 ENSMUST00000093785 ENSMUST00000027538 ENSMUST00000086843 ENSMUST00000038627 ENSMUST00000077798 ENSMUST00000168266 ENSMUST00000054780 ENSMUST00000112032 ENSMUST00000071554 ENSMUST00000112020

Gene Information

Description Not Available

Gene Symbol:   Klra10
Gene Name:   Killer cell lectin-like receptor subfamily A, member 10
Aliases:   Ly49J, Ly49i2
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000072718
Entrez:   16628
Chromosome Mapping:   6 F3|6 62.7 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.340000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download