PrimePCR™ SYBR® Green Assay: Gm5868, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0035010

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gm5868, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gm5868, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0005017
Assay Design:   Exonic
Chromosome Location:   5:72586253-72586400question
Amplicon Length:   118
Splice Variants Targeted:   ENSMUST00000030980 ENSMUST00000021278 ENSMUST00000161455 ENSMUST00000094251 ENSMUST00000040398 ENSMUST00000066560 ENSMUST00000040531 ENSMUST00000165810 ENSMUST00000043152 ENSMUST00000048015 ENSMUST00000106732 ENSMUST00000034464 ENSMUST00000020315 ENSMUST00000126373 ENSMUST00000030669 ENSMUST00000025815 ENSMUST00000034644 ENSMUST00000038816 ENSMUST00000031124 ENSMUST00000093945

Gene Information

Description Not Available

Gene Symbol:   Gm5868
Gene Name:   Predicted gene 5868
Aliases:   EG545758
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000060204
Entrez:   545758
Chromosome Mapping:   5 C3.2|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.970000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download