PrimePCR™ SYBR® Green Assay: Scand1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Scand1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Scand1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0004294
Assay Design:   Exonic
Chromosome Location:   2:156311877-156312053question
Amplicon Length:   147
Splice Variants Targeted:   ENSMUST00000042850 ENSMUST00000087951 ENSMUST00000116434 ENSMUST00000070785 ENSMUST00000045228 ENSMUST00000064371 ENSMUST00000121198 ENSMUST00000110434 ENSMUST00000079125 ENSMUST00000147005 ENSMUST00000166315 ENSMUST00000019660 ENSMUST00000110963 ENSMUST00000110962 ENSMUST00000066617

Gene Information

Description Not Available

Gene Symbol:   Scand1
Gene Name:   SCAN domain-containing 1
Aliases:   2310003H23Rik, Leap1, PGC-2, Ppargc2
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000046229
Entrez:   19018
Chromosome Mapping:   2|2 H2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.260000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download